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AssigneesCentre National De La Recherche Scientifique, Institut National de Recherche Agronomique, Institut National Des Sciences Appliquees De Toulouse
InventorsPierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
An isolated nucleotide sequence consisting essentially of a nucleotide sequence of SEQ ID NO:
A method of preparing a polypeptide having dextransucrase activity encoded by the polynucleotide sequence of SEQ ID NO:
A method for producing dextrans or isomalto-oligosaccharides with a controlled molar mass, comprising reacting with a polypeptide comprising the amino acid SEQ ID NO:
An isolated polynucleotide sequence encoding a protein comprising SEQ ID NO:
Dependencies are inferred from the claim text.