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AssigneeIogen Energy Corporation
InventorsJohn Tomashek, James Lavigne, Annie Tremblay, Patrick St-Pierre
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A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
A modified Family 6 glycosidase comprising an amino acid substitution at position 367 selected from the group consisting of W367G, W367A, W367C, W367N, W367R, W367S, W367T, and W367V, said position determined from alignment of a parental Family 6 glycosidase amino acid sequence with a Trichoderma reesei Cel6A amino acid sequence as defined in SEQ ID NO:
A modified Family 6 glycosidase comprising amino acid substitution selected W367G, said position determined from alignment of a parental Family 6 glycosidase amino acid sequence with a Trichoderma reesei Cel6A amino acid sequence as defined in SEQ ID NO:
A isolated Family 6 glycosidase comprising amino acid sequence TrCel6A-W367G-S413P (SEQ ID NO:
Dependencies are inferred from the claim text.