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US 8,263,361 B2Granted
Stabilized hematoxylin
AssigneeVentana Medical Systems, Inc.
InventorsJerome W. Kosmeder, Christopher Bieniarz, Penny Towne, Linda Willoughby Kivi
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Abstract
A stabilized hematoxylin composition is disclosed that includes one or both of a host compound and an antioxidant. The disclosed composition exhibits sufficient stability to be utilized in an automated staining process without undue degradation prior to use of the composition to stain a biological sample. Methods of using and making the stabilized composition also are disclosed.
Claims33
A stabilized hematoxylin composition for staining of a tissue or cytology sample, comprising:
- a solvent;
- hematoxylin;
- an amount of a chemical oxidant sufficient to convert at least a portion of the hematoxylin to hematein;
- a mordant;
- a host compound;
- and
- an antioxidant;
- wherein the host compound is selected from the group consisting of amylose, a cyclodextrin, a cryptand, a cryptophane, a cavitand, a crown ether, a dendrimer, a nanotube, a calixarene, a valinomycin, a nigericin, and combinations thereof.
5 more elements
Dependencies are inferred from the claim text.