Preparation of biomolecules
AssigneeGE Healthcare Bio-Science AB
InventorsElisabeth Hallgren, Ronnie Palmgren, Linda Svensson
Life of this patent
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Abstract
The present invention relates to a method of separating a target from a liquid, which includes providing a polymer in an aqueous liquid, which polymer comprises at least one hydrophobic portion; contacting the polymer-containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained. One phase is polymer-rich and contains target(s) and another phase is polymer-poor. By either maintaining the stimulus, or applying a different stimulus and maintaining it, the polymer-rich phase is transformed into a substantially solid phase.
Claims20
A method of separating at least one target from a liquid, which comprises:
- (a) providing at least one responsive polymer in an aqueous liquid, wherein the polymer comprises at least one hydrophobic portion;
- (b) contacting the aqueous liquid of (a) with the liquid comprising the target(s) to form a mixture thereof;
- (c) applying at least one first stimulus to the mixture resulting from (b) and maintaining it until a reversible phase separation is obtained, wherein one phase is a polymer-rich phase which comprises at least one target and the other phase is a polymer-poor phase;
- and
- (d) applying at least one second stimulus, which is different from the first stimulus, to the polymer-rich phase and maintaining it, until the polymer-rich phase has transformed into a substantially solid phase comprising ≦50% aqueous liquid, based on the total contents of polymer, target and liquid of the substantially solid phase, and
- (e) isolating the substantially solid phase comprising the target(s).
Dependencies are inferred from the claim text.