Nucleic acid isolation
AssigneeInvitrogen Dynal AS
InventorsJack Andreassen, Lars Korsnes, Stine Bergholtz
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Abstract
The present invention relates to a method of isolating nucleic acid from a blood sample, said method comprising: (a) selectively isolating leucocytes from said sample by binding said leucocytes to a solid support by means of a binding partner specific for leucocytes; (b) lysing said isolated leucocytes; and (c) binding nucleic acid released from said lysed cells to said solid support. Kits for isolating nucleic acid from samples form further embodiments of the invention.
Claims25
A method of isolating nucleic acid from a blood sample, said method comprising:
- (a) selectively isolating leucocytes from said sample by binding said leucocytes to a first solid support by means of at least two different binding partners specific for leucocytes;
- (b) lysing said isolated leucocytes;
- (c) binding nucleic released from said lysed leucocytes to said first solid support;
- and
- (d) contacting the nucleic acid of step (c) with an additional amount of a second solid support, and binding the nucleic acid to said second solid support;
- wherein the method is performed using an automated system for handling the first solid support and second solid support during cell lysis, nucleic acid binding or both, and wherein the first solid support and second solid support are transferred from a first tube to a second tube by the automated system, wherein the at least two different binding partners recognize or are capable of binding specifically to CD45 and CD15, and wherein the first solid support comprises polymeric particles.
A method of isolating nucleic acid from a cell sample, said method comprising:
- (a) selectively isolating cells from said sample by binding said cells to a first solid support by means of at least two different binding partners specific for said cells;
- (b) lysing said isolated cells;
- (c) binding nucleic acid released from said lysed cells to said first solid support;
- and
- (d) contacting the nucleic acid of step (c) with an additional amount of a second solid support, and binding said nucleic acid to said second solid support;
- wherein the method is performed using an automated system for handling of the first solid support and second solid support during cell lysis, nucleic acid binding or both, and wherein the first solid support and second solid support are transferred from a first tube to a second tube by the automated system, wherein the at least two different binding partners recognize or are capable of binding specifically to CD45 and CD15, and wherein the first solid support comprises polymeric particles.
Dependencies are inferred from the claim text.