Variants of group 1 allergens from Poaceae having reduced allergenicity and maintained T-cell reactivity
AssigneeMerck Patent GmbH
InventorsHelmut Fiebig, Martin Wald, Andreas Nandy, Helga Kahlert, Bernahrd Weber, Oliver Cromwell
Life of this patent
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Abstract
The present invention relates to the preparation and use of variants of the group 1 allergens of the Poaceae (sweet grasses) which are characterised by reduced IgE reactivity compared with the known wild-type allergens and at the same time by substantially maintained reactivity with T-lymphocytes. These hypoallergenic allergen variants can be employed for the specific immunotherapy (hyposensitisation) of patients having grass pollen allergy or for the preventive immunotherapy of grass pollen allergies.
Claims16
A recombinant variant of Phleum pratense Phl p 1 polypeptide, comprising substitution or deletion of cysteines at amino acid positions 41, 57, 69, 72, 77, 83 and 139 in SEQ ID NO:
- 2.
A variant of Phleum pratense Phl p 1 polypeptide comprising deletion of at least one region or a combination of regions corresponding to amino acids 1-6, 1-30, 92-104, 115-119, 175-185 and 213-220 in the mature Phl p 1 polypeptide sequence set forth in SEQ ID NO:
- 2.
A recombinant variant of Phleum pratense Phl p 1 polypeptide comprising deletion of at least one region or a combination of regions corresponding to amino acids 1-6, 1-30, 92-104, 115-119, 175-185 and 213-220 of the primary sequence of the mature Phl p 1 polypeptide sequence set forth in SEQ ID NO:
- 2 and further comprising substitution of cysteines at amino acid positions 41, 57, 69, 72, 77, 83 and 139 in said SEQ ID NO:
- 2.
A deletion variant of Phleum pratense polypeptide, which comprises the polypeptide sequence set forth in SEQ ID NO:
- 6, SEQ ID NO;
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16 or SEQ ID NO:
- 18.
Dependencies are inferred from the claim text.