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US 8,262,912 B1Granted
Isolated bioactive compounds and method of use
AssigneeTenfold Technologies, LLC
InventorsDavid P. Lanciault, Robert N. Ames
Life of this patent
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Abstract
Bioactive compounds extracted from a fermentation broth created by a unique microbial community during a fermentation process and fractionated into several fractions based on size. Four of the resulting fractions stimulate growth of plants that are planted in soil to which the fractions have been applied. The fractions can be applied to the soil individually or combined together prior to application. In another embodiment, the unique fractions can be freeze dried or spray dried.
Claims22
A method of isolating bioactive compounds comprising:
- apportioning an amount of a fermentation extract solution;
- removing biological matter resulting in a clarified liquid;
- mixing the clarified liquid with ammonium sulfate solution resulting in a precipitate;
- isolating the precipitate by centrifugation;
- redissolving the precipitate in a phosphate buffer;
- fractionizing the redissolved precipitate into seven fractions, each fraction sequentially decreasing in compound size;
- removing a set of one or more chosen fractions of the seven fractions and,
- applying the set of one or more chosen fractions to a portion of soil media adjacent a plant,
- wherein a plurality of the seven fractions are capable of stimulating plant growth in a plant that is in the treated soil.
6 more elements
Dependencies are inferred from the claim text.