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US 8,257,964 B2Granted
Microwell cell-culture device and fabrication method
AssigneeCell ASIC
InventorsPaul Ju-Sung Hung, Philip Janmin Lee
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Abstract
Methods for fabricating microfluidics devices, microfluidics cell-culture device and systems having a substrate, a microchannel through which liquid can be moved from one station to another within the device, the fabrication methods using semi-rigid materials during molding that are incorporated into the final device.
Claims22
A method of molding an elastomeric part comprising:
- (a) preparing a mold having a surface coating;
- (b) dispensing an elastomer precursor over the coated mold;
- (c) placing a semi-rigid sheet on top of the elastomer precursor such that a first side of the sheet is in contact with the elastomer precursor;
- (d) at least partially curing the elastomer precursor;
- (e) permanently joining the semi-rigid sheet to the elastomer precursor;
- (f) removing the joined semi-rigid sheet and the at least partially cured elastomer from the mold;
- (g) forming one or more access holes through the semi-rigid sheet and into the molded elastomer;
- (h) bonding the surface of the elastomer opposite the semi-rigid sheet to a substrate;
- and
- (g) attaching a second side of the semi-rigid sheet to a structure for accessing the molded elastomer.
7 more elements
Dependencies are inferred from the claim text.