Ketoreductase polypeptides for the production of azetidinone
AssigneeCodexis, Inc.
InventorsOnorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
Life of this patent
Estimating the term…
Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Claims17
An engineered ketoreductase polypeptide capable of converting the substrate, methyl-2-benzamidomethyl-3-oxobutyrate, to the product, 2R,3R-methyl-2-benzamidomethyl-3-hydroxybutyrate, wherein the ketoreductase comprises an amino acid sequence that is at least 90% identical to a reference sequence of SEQ ID NO:
- 66 and which comprises a set of mutations as compared to SEQ ID NO:
- 4 of any one of the polypeptide sequences of SEQ ID NO:
- 64, 66, 68, 70, 72, 74, 76, 78, 80, or 82.
Dependencies are inferred from the claim text.