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US 8,257,925 B2Granted
Method for detecting the presence of a single target nucleic acid in a sample
AssigneesApplied Biosystems, LLC, The United States of America as represented Department of Health & Human Services
InventorsJames F. Brown, Jonathan E. Silver
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Abstract
A miniaturized assembly is provided whereby a fluid sample can be divided into a plurality of sample portions in retaining wells and the sample fluid can be displaced from open ends of the wells while simultaneously being sealed in the wells. A method of dividing a fluid sample using the assembly is also provided.
Claims15
A method for detecting the presence of a single target nucleic acid molecule in a sample, said method comprising:
- providing a sample containing at least one target nucleic acid molecule to be amplified and constituents for enabling amplification of the target nucleic acid molecule;
- loading the sample into a sample chamber, said sample chamber retaining a reaction product of amplification of a single target nucleic acid molecule of said sample such that a reaction product of the amplification of the single target nucleic acid molecule attains a detectable concentration within a portion of said sample chamber after a single round of amplification when subjected to a homogeneous amplification assay;
- subjecting the sample in said sample chamber to a homogeneous amplification assay including a single round of amplification under conditions such that amplification of said at least one target nucleic acid molecule occurs and the reaction product of the amplification of a single target nucleic acid molecule of said sample attains a detectable concentration within said portion of said sample chamber after said single round of amplification;
- and
- detecting the reaction product of said single target nucleic acid molecule after said single round of amplification.
2 more elements
Dependencies are inferred from the claim text.