Pseudoinfectious flavivirus and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsPeter W. Mason, Elena Frolova, Ilya Frolov
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Abstract
The present invention discloses a replication-deficient pseudoinfective virus belonging to the Flaviviridae family that lack the capsid gene, where the replication-deficient pseudoinfective virus propagates only in cells expressing the capsid or capsid, prM and envelope protein of the flavivirus. The present also discloses the method of producing such viruses on a large scale and the use of these pseudoinfective viruses as vaccines for preventing diseases caused by infections of humans or animals by the viruses belonging to this family.
Claims25
A replication-deficient pseudoinfectious virus comprising:
- a West Nile or Yellow Fever deletion mutant genome comprising a deletion within the nucleotide sequence encoding amino acids 26 to 100 of the capsid protein, wherein the deletion mutant genome cannot produce capsid-containing viral particles in a cell that does not express a capsid protein, wherein the deletion does not disrupt the maturation of the prM protein or the RNA sequence required for genome cyclization, and a complementary capsid protein.
A cell culture system comprising:
- a West Nile or Yellow Fever deletion mutant genome comprising a deletion within the nucleotide sequence encoding amino acids 26 to 100 of the capsid protein, wherein the deletion mutant genome cannot produce capsid-containing viral particles in a cell that does not express a capsid protein, and wherein the deletion does not disrupt the maturation of the prM protein or the RNA sequence required for genome cyclization;
- the deletion mutant genome being inside a cell that expresses a complementary capsid protein, wherein the cell does not express prM or envelope proteins.
A method of producing a replication-deficient pseudoinfectious virus comprising:
- providing a West Nile or Yellow Fever deletion mutant genome comprising a deletion within the nucleotide sequence encoding amino acids 26 to 100 of the capsid protein, wherein the deletion mutant genome cannot produce capsid-containing viral particles in a cell that does not express a capsid protein, wherein the deletion does not disrupt the maturation of the prM protein or the RNA sequence required for genome cyclization;
- providing a cell that expresses a complementary capsid protein;
- inserting the deletion mutant genome into the cell;
- and
- culturing the cell to produce a replication-deficient pseudoinfectious virus.
Dependencies are inferred from the claim text.