Identification of a DNA variant associated with adult type hypolactasia

A composition comprising:

• (a) a first purified or isolated nucleic acid molecule consisting of a fragment of SEQ ID NO:3 or a fragment of the complement of SEQ ID NO:
• 3, and optionally a label, wherein said fragment consists of (i) from 14 to 30 consecutive nucleotides of SEQ ID NO:3 which includes position 324 of SEQ ID NO:3, or (ii) from 14 to 30 consecutive nucleotides of the complement of SEQ ID NO:3 which includes position 324 of said complement of SEQ ID NO:
• 3;
• and
• (b) a second purified or isolated nucleic acid molecule consisting of a fragment of SEQ ID NO:5 or a fragment of the complement of SEQ ID NO:
• 5, and optionally a label, wherein said fragment consists of (i) from 14 to 30 consecutive nucleotides of SEQ ID NO:5 which includes position 324 of SEQ ID NO:5, or (ii) from 14 to 30 consecutive nucleotides of the complement of SEQ ID NO:5 which includes position 324 of said complement of SEQ ID NO:
• 5.

A method for testing for the presence of or predisposition to adult-type hypolactasia in a subject, said method comprising:

• (a) contacting a nucleic acid obtained from said subject with a composition comprising:
• (i) a first purified or isolated nucleic acid molecule consisting of a fragment of SEQ ID NO:3 or a fragment of the complement of SEQ ID NO:
• 3, and optionally a label, wherein said fragment consists of (i) from 14 to 30 consecutive nucleotides of SEQ ID NO:3 which includes position 324 of SEQ ID NO:3, or (ii) from 14 to 30 consecutive nucleotides of the complement of SEQ ID NO:3 which includes position 324 of said complement of SEQ ID NO:
• 3;
• and
• (ii) a second purified or isolated nucleic acid molecule consisting of a fragment of SEQ ID NO:5 or a fragment of the complement of SEQ ID NO:
• 5, and optionally a label, wherein said fragment consists of (i) from 14 to 30 consecutive nucleotides of SEQ ID NO:5 which includes position 324 of SEQ ID NO:5, or (ii) from 14 to 30 consecutive nucleotides of the complement of SEQ ID NO:5 which includes position 324 of said complement of SEQ ID NO:
• 5;
• (b) detecting the presence or absence of hybridization between said first and second nucleic acid molecules with said nucleic acid obtained from said subject;
• and
• (c) determining the presence of or predisposition to adult-type hypolactasia in said subject when the presence of hybridization between said second nucleic acid molecule with said nucleic acid obtained from said subject is detected in the absence of hybridization between said first nucleic acid molecule and said nucleic acid obtained from said subject.