Method of inducing memory B cell development and terminal differentiation
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsCatherine Rachel Ettinger, Peter E. Lipsky, Warren J. Leonard, Rosanne Spolski, Herbert C. Morse, III
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Abstract
The invention provides a method for promoting differentiation of a mature naïve B cell or a B cell progenitor into a memory B cell or a plasma cell. The method comprises (a) contacting a population of cells comprising a mature naïve B cell or a B cell progenitor with an agent that activates at least one of JAK1, JAK3, STAT3, STAT5A or STAT5B; wherein the population of cells optionally is contacted with an antigen, and (b) isolating the memory B cell or plasma cell.
Claims8
A method for promoting differentiation of at least one mature naïve B cell and/or B cell progenitor into one or more memory B cells and/or plasma cells, the method comprising:
- (a) contacting a population of cells comprising mature naïve B cells and/or B cell progenitors with an IL-21 polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1, wherein the population of cells is contacted with an antigen, thereby promoting differentiation of at least one mature naïve B cell and/or B cell progenitor in the population of cells into one or more memory B cells and/or plasma cells;
- and
- (b) isolating memory B cells and/or plasma cells from the population of cells.
A method for promoting differentiation of at least one mature B cell and/or B cell progenitor into one or more memory B cells and/or plasma cells, the method comprising:
- (a) contacting a population of cells comprising mature B cells and/or B cell progenitors with an IL-21 polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1, wherein the population of cells is contacted with an antigen, thereby promoting differentiation of at least one mature B cell and/or B cell progenitor in the population of cells into one or more memory B cells and/or plasma cells;
- and
- (b) isolating memory B cells and/or plasma cells from the population of cells.
Dependencies are inferred from the claim text.