Penicillin-G acylases
AssigneeCodexis, Inc.
InventorsBehnaz Behrouzian, Anke Krebber, Emily Mundorff
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Abstract
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Claims6
An engineered penicillin G acylase (PGA) capable of cleaving substrate 6-nitro-3-(phenylacetamido)benzoic acid (NIPAB) to product 5-amino-2-nitrobenzoic acid, wherein the PGA comprises an α-chain sequence having at least 85% identity to SEQ ID NO:179 and an β-chain sequence having at least 85% identity to SEQ ID NO:180, wherein the polypeptide has improved activity as compared to the naturally occurring PGA of Kluyvera citrophila, wherein said engineered penicillin G acylase comprises SEQ ID NO:8.
A composition comprising:
- (i) a penicillin G of structural formula (I), a 6-amino penicillanic acid of structural formula (II), and/or a phenylacetic acid of structural formula (III);
- and (ii) an engineered PGA comprising SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.