Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
AssigneeCytori Therapeutics, Inc.
InventorsMarc H. Hedrick, John K. Fraser
Life of this patent
Estimating the term…
Abstract
Methods of treating patients for conditions such as breast augmentation, soft tissue defects, and urinary incontinence, are described. The methods include removing adipose tissue from a patient, processing a portion of the adipose tissue to obtain a substantially isolated population of cells comprising disaggregated adipose-derived stem cells, mixing the concentrated population of adipose-derived cells comprising disaggregated adipose-derived cells with a portion of unprocessed, intact, non-disaggregated adipose tissue to form a composition, and administering the composition to the patient from which the adipose tissue was removed.
Claims23
A method of augmenting autologous fat transfer to a subject, comprising:
- removing a first portion of adipose tissue that comprises a cell population comprising adipose-derived stem cells from said subject;
- introducing the removed adipose tissue into a self-contained cell processing unit configured to maintain a closed pathway;
- isolating said cell population that comprises adipose-derived stem cells from mature adipocytes and connective tissue present in the removed adipose tissue so as to obtain a substantially isolated cell population comprising disaggregated adipose-derived stem cells, while maintaining a closed pathway;
- concentrating said substantially isolated cell population comprising disaggregated adipose-derived stem cells so as to obtain a concentrated cell population that comprises disaggregated adipose-derived stem cells, while maintaining a closed pathway;
- mixing said concentrated cell population comprising adipose-derived stem cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments obtained from said subject, so as to obtain an adipose tissue composition comprising a mixture of said concentrated cell population comprising disaggregated adipose-derived stem cells and said unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments tissue;
- and
- reintroducing said adipose tissue composition into said subject.
Dependencies are inferred from the claim text.