Method for manufacturing glycoproteins having human-type glycosylation
AssigneePhyton Holdings, LLC
InventorsTatsuji Seki, Kazuhito Fujiyama
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Abstract
The present invention provides a method for manufacturing a glycoprotein having a human-type sugar chain comprising a step in which transformed plant cell is obtained by introducing to a plant cell the gene of glycosyltransfetase and the gene of an exogenous glycoprotein, and a step in which the obtained transformed plant cell is cultivated.
Claims14
A plant cell comprising a first exogenous gene encoding a glycosyltransferase and a second exogenous gene encoding an exogenous glycoprotein, in which the exogenous glycoprotein is expressed and the expressed glycoprotein comprises a human-type sugar chain, which has a galactose residue linked to an N-acetylglucosamine residue, wherein the glycosyltransferase is a galactosyltransferase.
A plant cell comprising a glycoprotein comprising a human-type sugar chain, comprising no fucose or xylose linked to one or more of the core sugar chain, the outer sugar chain and the terminal sugar chain.
A plant cell comprising an exogenous glycoprotein that comprises a galactose residue linked to an N-acetylglucosamine residue by a beta 1,4 linkage.
A plant cell comprising an exogenous glycoprotein that comprises a galactose residue which is added to the glycoprotein by a galactosyltransferase.
Dependencies are inferred from the claim text.