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AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
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The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
A non-naturally occurring microbial organism, comprising a 3-hydroxyisobutyric acid pathway, said microbial organism comprising exogenous nucleic acids encoding 3-hydroxyisobutyric acid pathway enzymes expressed in a sufficient amount to produce 3-hydroxyisobutyric acid, said 3-hydroxyisobutyric acid pathway comprising 4-hydroxybutyryl-CoA mutase and 3-hydroxyisobutyryl-CoA synthetase or 3-hydroxyisobutyryl-CoA hydrolase or 3-hydroxyisobutyryl-CoA transferase.
A non-naturally occurring microbial organism, comprising a 3-hydroxyisobutyric acid pathway, said microbial organism comprising at least one exogenous nucleic acid encoding a 3-hydroxyisobutyric acid pathway enzyme expressed in a sufficient amount to produce 3-hydroxyisobutyric acid, said at least one exogenous nucleic acid encoding 4-hydroxybutyryl-CoA mutase.
A non-naturally occurring microbial organism, comprising a 3-hydroxyisobutyryl-CoA pathway, said microbial organism comprising at least one exogenous nucleic acid encoding a 3-hydroxyisobutyryl-CoA pathway enzyme expressed in a sufficient amount to produce 3-hydroxyisobutyryl-CoA, said at least one exogenous nucleic acid encoding 4-hydroxybutyryl-CoA mutase.
Dependencies are inferred from the claim text.