Method for detection of microorganism and kit for detection of microorganism
AssigneeMorinaga Milk Industry Co., Ltd.
InventorsShinichi Yoshida, Takashi Soejima
Life of this patent
Estimating the term…
Abstract
A kit is disclosed for preparing a measurement sample for detecting live cells, injured cells, VNC cells and dead microorganism cells in a test sample by the following steps: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.
Claims4
A kit for use in a method for preparing a measurement sample for detecting live cells, injured cells and dead cells of a microorganism in a test sample by flow cytometry, which comprises the following elements:
- an enzyme selected from lipolytic enzymes and proteases, which has an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample,
- a topoisomerase poison selected from the group consisting or amsacrine, camptothecin, doxorubicin, ellipticine, etoposide, mitoxantrone, saintopin, topotecan CP-115,953 and ethidium monoazide and/or a DNA gyrase poison selected from the group consisting of ciprofloxacin, ofloxacin, enoxacin, pefloxacin, fleroxacin, norfloxacin, nalidixic acid, oxolinic acid and piromidic acid, and
- a first stain agent which stains live cells in different color from injured cells and dead cells, and a second stain agent which stains live cells and injured cells in different color from dead cells.
Dependencies are inferred from the claim text.