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US 8,236,527 B2Granted
Recombinant production of authentic human proteins using human cell expression systems
AssigneeHumanzyme Limited
InventorsRidong Chen, Soon Seog Jeong, Hui Feng
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Abstract
The present invention relates to novel expression cassettes and vectors for efficiently producing authentic recombinant human proteins from stable cultures of novel human cell lines, the authentic recombinant proteins produced therefrom, and antibodies raised against those authentic recombinant proteins.
Claims18
An expression vector, comprising a cassette which comprises the following operably linked expression elements:
- (A) a human cytomegalovirus immediate early enhancer element;
- (B) a functional human beta-actin promoter;
- (C) a human globin gene intron;
- and
- (D) a human signal peptide consisting of a immunoglobulin superfamily 8 signal peptide, or an alpha-fibrinogen signal peptide.
2 more elements
A stable human cell line called HZ-293TS deposited under and bearing the ATCC biological deposit accession number of PTA-10165.
Dependencies are inferred from the claim text.