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US 8,236,511 B2Granted
Quantitation of cellular DNA and cell numbers using element labeling
AssigneeDvs Sciences Inc.
InventorsOlga Ornatsky, Vladimir Baranov
Life of this patent
Estimating the term…
Abstract
Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers.
Claims9
A method to quantify the number of cells in a sample comprising cells, the method comprising:
- (a) culturing the sample with an element to passively accumulate the element in the cells in the sample;
- (b) washing the cells to remove non-accumulated element;
- (c) measuring the element in a known quantity of the cells by elemental analysis to determine the amount of accumulated element per cell;
- (d) measuring the element in the sample by elemental analysis;
- and
- (e) quantifying the number of cells in the sample by dividing (d) by (c).
3 more elements
A method to quantify antigen content per cell in a cell sample wherein the cells of the sample comprise an antigen, comprising:
- (a) culturing the cells of the sample in the presence of a first element wherein the cells passively accumulate the first element;
- (b) calculating the number of cells in the sample;
- (c) removing non-accumulated first element;
- (d) incubating the cells with antigen specific affinity products labeled with a second element;
- (e) removing unbound affinity products;
- (f) simultaneously measuring the passively accumulated first element and the bound affinity product labeled with a second element tag by elemental analysis;
- and
- (g) calculating the antigen content per cell.
5 more elements
Dependencies are inferred from the claim text.