Identification of antigenic peptides from multiple myeloma cells
AssigneeJanssen Pharmaceutica NV
InventorsZeling Cai, Wei-Xing Shi, Xuejun P. Liu, Jiejun Wu
Life of this patent
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Abstract
Multiple myeloma (MM) is a clonal B cell malignancy and remains essentially incurable by conventional anti-tumor therapy. Patients with MM have a median survival of only three years. MM is characterized by proliferation and accumulation of mature plasma cells in the bone marrow (BM) leading to bone destruction, BM failure, anemia, and reduced immune function. The identification of MHC Class I, HLA-A2, associated peptides presented on multiple myeloma cells is an important step in developing immunotherapies for MM. Presented here are methods for creating activated T lymphocytes that are cytotoxic to both peptide loaded T2 target cells and multiple myeloma cell lines.
Claims7
A method for creating activated T lymphocytes for administration to a patient diagnosed with multiple myeloma, the method comprising the steps of:
- (a) preparing antigenic peptide loaded Drosophila artificial antigen presenting cells (aAPCs) by loading Drosophila aAPCs with at least one antigenic peptide, wherein the antigenic peptide consisting of an amino acid sequence selected from the group consisting of:
- SLVLNLLEL (SEQ ID NO:3), KNPVLLKIL (SEQ ID NO:7), NLLPKLHVV (SEQ ID NO:9), FLLPHPGLQV (SEQ ID NO:10), LLNMPPAHLK (SEQ ID NO:11), TLVDLPGMTKV (SEQ ID NO:13), TLIDLPGITRV (SEQ ID NO:14), ALNEEAGRLLL (SEQ ID NO:15), LSLDSSLSSLL (SEQ ID NO:17), LLLDVAYGAVQA (SEQ ID NO:22), FLASESLLKGAL (SEQ ID NO:23), LVLNLLE (SEQ ID NO:32), TLVDLPGM (SEQ ID NO:40), TLVDLP (SEQ ID NO:42), LVDLPGM (SEQ ID NO:45), IDLPGITR (SEQ ID NO:61), LIDLPGI (SEQ ID NO:62), IDLPGIT (SEQ ID NO:63), WLTVLFIFRI (SEQ ID NO:66), LVYLGHVIYL (SEQ ID NO:67), KLHDAIVEVV (SEQ ID NO:68), KLHDAIVEV (SEQ ID NO:69), FVPEVSFEL (SEQ ID NO:70), and FQMEQIVYC (SEQ ID NO:72);
- (b) isolating T lymphocytes from the patient;
- (c) contacting said T lymphocytes with said antigenic peptide loaded Drosophila aAPCs;
- (d) generating activated T lymphocytes, wherein the activated T lymphocytes are cytotoxic to multiple myeloma cancer cells;
- and,
- (e) collecting the activated T lymphocytes for administration back to the patient.
Dependencies are inferred from the claim text.