Tetracycline-sensitive bifidobacteria strains
AssigneeChr. Hansen A/S
InventorPer Stroeman
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Abstract
Many probiotic Bifidobacteriacea contains an active tetW that renders the cells resistant to tetracycline. This presents a theoretical risk of a horizontal transfer of functional antibiotic genes. The present invention relates to novel tetracycline-sensitive strains of the well-known probiotic Bifidobacterium animalis subsp. lactis strain BB-12® and the use of such novel strains for the preparation of a food or feed product or a dosage form comprising viable organisms.
Claims3
An isolated Bifidobacterium animalis subspecies lactis strain containing an inactivated tetW on its chromosome, wherein said strain is selected from the group consisting of:
- (i) the Bifidobacterium animalis subspecies lactis strains having the registration number DSM 18735 (CHCC 9870) or DSM 18776 (CHCC 9884), and
- (ii) a mutant strain which is obtained by using either of the deposited strains DSM 18735 (CHCC 9870) and DSM 18776 (CHCC 9884) as starting material and said mutant strain has a minimum inhibitory concentration of 1.5 microgram tetracycline/ml or less, wherein
- the tetW gene of said mutant comprises either the sequence of SEQ ID NO:
- 3 [GAA AAT ACA AGC] or
- the sequence of SEQ ID NO:
- 6 [GAA TAC AGG CTT].
Dependencies are inferred from the claim text.