Kit for detection of telomerase reverse transcriptase nucleic acids
AssigneesGeron Corporation, The Regents of the University of Colorado
InventorsThomas R. Cech, Joachim Lingner, Toru M. Nakamura, Karen B. Chapman, Gregg B. Morin, Calvin B. Harley, William H. Andrews
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.
Claims16
A kit for detecting a nucleic acid that encodes human telomerase reverse transcriptase (hTRT), or fragment thereof, in a sample, the kit comprising a pair of amplification primers, said pair comprising a first amplification primer at least 10 nucleotides in length that hybridizes specifically to a DNA having the sequence of SEQ. ID NO:
- 1 or the complement thereof at 5° C. to 25° C. below Tm in aqueous solution at 1 M NaCl but under the same reaction conditions does not hybridize to a DNA having the sequence of SEQ. ID NO:8 or the complement thereof;
- and a second amplification primer at least 10 nucleotides in length that hybridizes specifically to a DNA having the sequence of SEQ. ID NO:
- 1 or the complement thereof at 5° C. to 25° C. below Tm in aqueous solution at 1 M NaCl.
A kit for detecting a nucleic acid that encodes human telomerase reverse transcriptase (hTRT), or fragment thereof, in a sample, the kit comprising a pair of oligonucleotide amplification primers, said pair comprising a first amplification primer that comprises at least 10 consecutive nucleotides of SEQ. ID NO:1 or the complement thereof that are not contained in SEQ. ID NO:8 or its complement;
- and a second amplification primer that comprises at least 10 consecutive nucleotides of SEQ. ID NO:1 or the complement thereof.
A pair of oligonucleotide primers for amplifying a nucleic acid that encodes human telomerase reverse transcriptase (hTRT) or fragment thereof, wherein each primer comprises at least 10 consecutive nucleotides of SEQ. ID NO:
- 1 or the complement thereof, and at least one primer comprises at least 10 consecutive nucleotides of SEQ ID NO:1 or the complement thereof that are not contained in SEQ. ID NO:8 or the complement thereof.
Dependencies are inferred from the claim text.