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US 8,222,007 B2Granted
L-glutamic acid producing bacterium and a method for producing L-glutamic acid
AssigneeAjinomoto Co., Inc.
InventorsYoshihiko Hara, Hiroshi Izui
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Abstract
The present invention describes an L-glutamic acid-producing bacterium which belongs to the genus Pantoea, Enterobacter, Klebsiella or Erwinia, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.
Claims6
A method for producing L-glutamic acid comprising:
- A) culturing in a medium an L-glutamic acid-producing bacterium which belongs to a genus of bacteria selected from the group consisting of Pantoea, Enterobacter, Klebsiella, and Erwinia, and
- B) collecting L-glutamic acid from the medium,
- wherein the bacterium has been modified by gene recombination so that the rpoS gene is inactivated;
- wherein the rpoS gene encodes a protein selected from the group consisting of:
- (A) the protein comprising the amino acid sequence of SEQ ID NO:
- 2, and
- (B) the protein comprising the amino acid sequence of SEQ ID NO:
- 2, but which includes substitutions, deletions, insertions, or additions of one to 5 amino acid residues, and which has sigma S factor activity of RNA polymerase.
5 more elements
Dependencies are inferred from the claim text.