Liquid phase peptide synthesis of KL-4 pulmonary surfactant
AssigneeOrtho Pharmaceutical Corporation
InventorsAhmed F. Abdel-Magid, Urs Eggmann, Cynthia Anne Maryanoff, Adrian Thaler, Frank J. Villani
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Abstract
The invention relates to improved liquid phase processes for the preparation of the 21 residue protein component, (Lys-Leu4)4-Lys, of the pulmonary surfactant KL-4. These process are amenable to large scale synthesis and one process employs a method of saponifying an ester which reduces the inherent racemization of the α-carbon.
Claims2
A liquid phase method of deprotecting a peptide's ester protected carboxy group having an α chiral center which consists of reacting said peptide ester with a tetraalkylammonium hydroxide and water in an inert organic solvent at a low temperature to form the deprotected peptide's carboxy group.
Dependencies are inferred from the claim text.