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US 8,211,699 B2Granted
Methods for culturing pluripotent stem cells in suspension using ERBB3 ligands
AssigneeViaCyte, Inc.
InventorsAllan J. Robins, Thomas C. Schulz
Life of this patent
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Abstract
The present invention relates to cell culture methods and compositions that are essentially serum-free and comprise a basal salt nutrient solution and an ErbB3 ligand.
Claims33
A method of expanding pluripotent human stem cells in suspension, said method comprising
- a) placing the pluripotent stem cells in a suspension of cell medium, the medium comprising a basal salt nutrient solution and an ErbB3 ligand, where the cell medium is essentially free of serum, wherein the pluripotent human stem cells can give rise to all three germ layers and wherein the ErbB3 ligand binds to an ErbB3 receptor, which in turn dimerizes to an ErbB2 receptor to form an ErbB2/ErbB3 heterodimer, thereby activating the tyrosine kinase activity of the ErbB2 receptor in the ErbB2/ErbB3 heterodimer;
- and
- b) maintaining the pluripotent stem cells in the suspension of culture medium and in appropriate culture conditions for more than one day, wherein the cell medium is changed periodically,
- wherein the pluripotent stem cells expand and do not differentiate.
1 more element
A method of expanding primate embryonic stem cells in suspension, said method comprising
- a) placing the primate embryonic stem cells in a suspension of cell medium, the medium comprising a basal salt nutrient solution and an ErbB3 ligand, where the cell medium is essentially free of serum;
- and
- b) maintaining the primate embryonic stem cells in the suspension of culture medium and in appropriate culture conditions for more than one day, wherein the cell medium is changed periodically,
- wherein the primate embryonic stem cells expand and do not differentiate.
1 more element
Dependencies are inferred from the claim text.