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US 8,206,915 B2Granted
Nucleic acid enzyme biosensors for ions
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Jing Li
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Abstract
Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes.
Claims22
A method of detecting the presence of an ion comprising:
- (a) contacting a nucleic acid enzyme, the enzyme dependent on the ion to produce a product from a substrate, with a sample suspected of containing an ion;
- and
- (b) measuring the product of the nucleic acid enzymatic reaction;
- wherein the ion is a metal ion that is not Mg2+, and
- the substrate comprises a fluorophore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore.
2 more elements
A biosensor comprising:
- (a) a nucleic acid enzyme dependent on an ion to produce a product from a substrate;
- and
- (b) the substrate;
- wherein the ion is not Mg2+, and
- the substrate comprises a fluorophore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore.
2 more elements
A composition comprising a nucleic acid enzyme linked to a fluorophore,
- wherein the nucleic acid enzyme is responsive to an ion selected from the group consisting of K+, Na+, Li+, Tl+, Ag+, Ca2+, Mn2+, Co2+, Ni2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Pt2+, Ra2+, Ba2+, Sr2+, Co3+, Cr3+, Ln3+, and Ce4+.
Dependencies are inferred from the claim text.