Microfluid mixer, methods of use and methods of manufacture thereof

A mixer comprising:

• a base plate;
• a first plate in intimate contact with a surface of the base plate;
• the intimate contact preventing a fluid loss from the base plate;
• the first plate comprising:
• a plurality of first ports for the entry of a first fluid and a second fluid;
• a mixing chamber in fluid communication with the plurality of first ports;
• a first heating element;
• an exit channel for removing an intimate mixture of the first fluid and the second fluid;
• a second plate disposed upon the first plate;
• the second plate having a first surface and a second surface;
• the first surface and the second surface being opposedly disposed;
• the first surface being in intimate contact with the first plate in a manner that prevents loss of any fluid introduced into the second plate;
• the second plate comprising:
• a plurality of second ports for the entry of a first fluid and a second fluid;
• the second ports being in fluid communication with the first ports;
• another mixing chamber in fluid communication with the mixing chamber and the plurality of second ports;
• a reciprocatory device in physical contact with second plate at the another mixing chamber;
• the reciprocatory device being operative to agitate the first fluid and the second fluid;
• and
• a second heating element;
• the second heating element being operative for creating a temperature gradient in the another mixing chamber;
• the intimate contact between the first plate and a surface of the base plate serving to prevent any loss of the first fluid and the second fluid from the mixer.