Nucleoside triphosphate derivative, nucleic acid probe, multilabeled nucleic acid probe, method for production of multilabeled nucleic acid probe, and method for detection of target nucleic acid

A nucleic acid probe, comprising:

• a plurality of nucleoside triphosphate derivatives,
• wherein each of the plurality of nucleoside triphosphate derivatives is represented by formula (5) below:
• wherein each of X and Y independently represents a bivalent linking group,
• wherein Z represents a substituent, and
• wherein B represents a hydrogen atom or a hydroxyl group.

A method for producing a multilabeled nucleic acid probe, the method comprising:

• providing either
• (1) a nucleic acid probe comprising plurality of nucleoside triphosphate derivatives, each of the plurality of nucleoside triphosphate derivatives having a glutamine residue, and a labeling compound comprising a lysine residue and a labeling portion, or
• (2) a nucleic acid probe comprising plurality of nucleoside triphosphate derivatives, each of the plurality of nucleoside triphosphate derivatives having a lysine residue, and a labeling compound comprising a glutamine residue and a labeling portion, and
• using a transglutaminase to bind the labeling compound to the nucleic acid probe,
• wherein said labeling compound is bound to the nucleic acid probe by covalent binding between the glutamine residue and the lysine residue.

A method for detecting a target nucleic acid, the method comprising:

• performing specific binding, via nucleic acid portions, of:
• a nucleic acid probe comprising a plurality of nucleoside triphosphate derivatives, where either (1) each of the plurality of nucleoside triphosphate derivatives have a glutamine residue, or (2) each of the plurality of nucleoside triphosphate derivatives have a lysine residue, and
• a target nucleic acid,
• subsequently introducing a plurality of labeling compounds which either (1) comprise a glutamine residue and a labeling portion, or (2) comprise a lysine residue and a labeling portion, then
• using a transglutaminase to react the labeling compounds with the nucleic acid probes, and
• detecting the bound nucleic acid probe via the labeling portions,
• wherein said labeling compounds are bound to the nucleic acid probe by covalent binding between the glutamine residue and the lysine residue.