Hydrolases, nucleic acids encoding them and methods for making and using them
AssigneeDsm IP Assets B.V.
InventorsTimothy Hitchman, Christopher L. G. Dayton, Katie A. Kline, Jonathan Lyon, Mark Wall, Nelson R. Barton
Life of this patent
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Abstract
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
Claims11
An isolated, synthetic, or recombinant polypeptide having palmitase activity comprising
- an amino acid sequence having at least 85% sequence identity to SEQ ID NO:2, and an amino acid residue modification of V163R, wherein the substrate specificity comprises increased hydrolysis of palmitic acid from an oil.
Dependencies are inferred from the claim text.