Compositions comprising cardiac stem cells overexpressing specific micrornas and methods of their use in repairing damaged myocardium
AssigneeNew York Medical College
InventorsToru Hosoda, Piero Anversa, Annarosa Leri, Jan Kajstura
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Abstract
The invention provides compositions comprising modified stem cells containing a transgene that affects the expression of at least one gene that inhibits or promotes cardiomyogenesis. In particular, the invention discloses compositions comprising cardiac stem cells, wherein said cardiac stem cells comprise a transgene encoding a microRNA. The compositions of the invention find use in the treatment of cardiovascular disorders, such as myocardial infarction. Methods of repairing damaged myocardium in a subject using the modified stem cells are also disclosed.
Claims22
A pharmaceutical composition comprising isolated adult cardiac stem cells and a pharmaceutically acceptable carrier, wherein said cardiac stem cells are lineage negative and c-kit positive and comprise a transgene encoding miR-499.
A method for regenerating damaged myocardium in a subject in need thereof comprising:
- (a) extracting cardiac stem cells from the subject, wherein said cardiac stem cells are lineage negative and c-kit positive;
- (b) incorporating a transgene into said extracted cardiac stem cells, wherein said transgene encodes miR-499;
- and
- (c) administering the cardiac stem cells from step (b) to an area of damaged myocardium in the subject, wherein the cardiac stem cells differentiate into mature, functional cardiomyocytes following administration, thereby regenerating the damaged myocardium.
Dependencies are inferred from the claim text.