Sesquiterpene synthase gene and protein
AssigneeUniversity of Kentucky Research Foundation
InventorsJoseph Chappell, Bryan Greenhagen
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Abstract
The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
Claims9
A method of producing valencene, comprising:
- a) reacting a heterologous polypeptide having valencene synthase enzymatic activity with farnesyl pyrophosphate under conditions in which the enzymatic activity catalyzes the formation of a valencene, wherein:
- the reaction of the polypeptide with farnesyl pyrophosphate is performed at pH between about 6.0 and 7.0;
- and
- the polypeptide comprises the sequence of amino acids set forth in SEQ ID NO:
- 4;
- and
- (b) isolating the valencene formed in step (a).
A method of producing valencene comprising the steps of:
- (a) reacting a heterologous polypeptide having valencene synthase enzymatic activity with farnesyl pyrophosphate under conditions in which the enzymatic activity catalyzes the formation of a valencene, wherein the polypeptide comprises the sequence of amino acids set forth in SEQ ID NO:4;
- and
- (b) isolating the valencene formed in step (a).
Dependencies are inferred from the claim text.