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US 8,187,862 B2Granted
Bioremediation methods
AssigneeCL Solutions, LLC
InventorsMichael T. Saul, Efrain Torres, Peter A. Vandenbergh
Life of this patent
Estimating the term…
Abstract
A method and composition for bioremediation of environmental material. In one embodiment, material to be bioremediated is debris is obtained from a metropolitan street cleaning operation. An environmentally safe all natural non-pathogenic microbial composition is used under conditions sufficient to bioremediate the material.
Claims20
A bioremediation method for street cleaning, comprising
- providing a composition consisting essentially of at least one genus of Pseudomonas to street sweeping debris collected in a street cleaning process, the composition provided under conditions sufficient to reduce a level of at least one contaminant in the debris, resulting in at least partially bioremediated debris, the composition selected from the group consisting of
- (a) a combination of Pseudomonas putida strains B and E,
- (b) a combination of Pseudomonas fluorencens strain F and Pseudomonas putida strain I,
- (c) a combination of Pseudomonas putida strains B and E and Pseudomonas fluorencens strain G,
- (d) Pseudomonas putida strain B,
- (e) Pseudomonas putida strain E,
- (f) Pseudomonas putida strain I,
- (g) Pseudomonas fluorencens strain F, and combinations thereof.
5 more elements
A bioremediation method for street cleaning comprising
- applying a composition, the composition consisting essentially of at least one Pseudomonas genus in a concentration ranging from about 1×106 cfu/ml to about 1×109 cfu/ml and at least one nutrient, to street cleaning debris collected from a metropolitan street cleaning process to be bioremediated resulting in a treated material, the composition applied under conditions sufficient to reduce a level of at least one contaminant in the treated material, the conditions selected from the group consisting of maintenance of about 10% to about 30% moisture, about 15% oxygen to about 21% oxygen, about 75° F. to about 95° F. temperature, and combinations thereof, for a time sufficient to result in a bioremediated material, wherein the at least one Pseudomonas genus is selected from the group consisting of
- (a) a combination of Pseudomonas putida strains B and E,
- (b) a combination of Pseudomonas fluorencens strain F and Pseudomonas putida strain I,
- (c) a combination of Pseudomonas putida strains B and E and Pseudomonas fluorencens strain G,
- (d) Pseudomonas putida strain B,
- (e) Pseudomonas putida strain E,
- (f) Pseudomonas putida strain I, and
- (g) Pseudomonas fluorencens strain F.
5 more elements
A bioremediation method for street cleaning comprising
- providing a composition consisting essentially of a combination of Pseudomonas putida strain B and Pseudomonas putida strain E, and optionally Pseudomonas fluorencens strain G, to street sweeping debris collected in a street cleaning process to result in a treated material, and
- determining bioremediation of the treated material by measuring degradation of petroleum contaminants in the treated material.
Dependencies are inferred from the claim text.