Microorganism producing inosine and method of producing inosine using the same
AssigneeCj Cheiljedang Corporation
InventorsYoung-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
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Abstract
The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
Claims4
A method for producing inosine by fermentation comprising:
- culturing a microorganism belonging to Corynebacterium ammoniagene in a culture medium to produce and accumulate inosine in the medium, and
- collecting inosine,
- wherein, said microorganism has inosine-producing ability and is modified to inactivate the gene encoding nucleoside hydrolase II having the nucleotide sequence represented by SEQ ID NO:
- 1, and is further modified to overexpress the gene endoding 5′-nucleotidase represented by SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.