Puumala virus full-length M segment-based DNA vaccines
AssigneeThe United States of America as represented by the Secretary of the Army
InventorJay Hooper
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Abstract
The invention contemplates a new synthetic, codon-optimized Puumala virus (PUUV) full-length M gene open reading frame (ORF) that encodes a unique consensus amino acid sequence. The PUUV ORF was cloned into a plasmid to form the first stable PUUV full-length M gene that elicits neutralizing antibodies. The gene can be engineered into a molecular vaccine system, and is useful to protect mammals against infection with Puumala virus.
Claims15
An isolated nucleic acid sequence comprising SEQ ID NO:1.
A recombinant DNA construct comprising:
- (i) a vector, and
- (ii) the DNA fragment comprising the nucleic acid sequence set forth in SEQ ID NO:1, operably linked to a promoter sequence.
A vaccine against infection with Puumala virus, said vaccine comprising a composition comprising inert particles and a nucleic acid coated onto the inert particles producing nucleic acid coated particles, said nucleic acid comprising a promoter operative in the cells of a mammal and SEQ ID NO:1.
A method for inducing a protective immune response against Puumala virus infection in a mammal, comprising the step of accelerating into epidermal cells of the mammal in vivo a composition comprising inert particles and a nucleic acid coated onto the inert particles producing nucleic acid coated particles, said nucleic acid comprising a promoter operative in the cells of a mammal and SEQ ID NO:1, such that said nucleic acid is expressed.
A DNA cassette comprising SEQ NO:1 linked to a promoter operable in a eukaryotic expression system.
Dependencies are inferred from the claim text.