Device for detection of target molecules and uses thereof

A device for detecting a target molecule comprising:

• a housing comprising:
• an inlet opening in fluid contact with a conjugate pad;
• a force member;
• a slidable locking member contacting the force member;
• an attachment member contacting the force member;
• a sliding button contacting the attachment member;
• and
• a detection membrane system comprising the conjugate pad, a test membrane, and an absorbent member,
• wherein at least a portion of the conjugate pad, test membrane, and absorbent member are substantially parallel to each other,
• wherein the force member contacts the detection membrane system and is capable of applying pressure substantially perpendicular to the detection membrane system, and
• wherein the sliding button moves the slidable locking member.