Composition comprising recombinant nucleic acid encoding Sox2, Oct-4, Nanog and Lin28
AssigneeWisconsin Alumni Research Foundation
InventorsJames A. Thomson, Junying Yu
Life of this patent
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Abstract
The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.
Claims16
A composition comprising recombinant nucleic acid encoding potency-determining factors SOX2, OCT-4, NANOG, and LIN28, the factor-encoding nucleic acid being operably linked to at least one heterologous promoter, wherein the composition induces reprogramming when introduced into a mammalian cell.
A transgenic mammalian euploid cell comprising a recombinant genetic construct encoding potency-determining factors SOX2, OCT-4, NANOG, and LIN28 that induces reprogramming of the mammalian cell.
A kit for cellular reprogramming, the kit comprising:
- nucleic acid encoding SOX2,
- nucleic acid encoding OCT-4;
- nucleic acid encoding NANOG;
- nucleic acid encoding LIN28 and a heterologous promoter operably linked to at least one of the nucleic acid encoding SOX2, nucleic acid encoding OCT-4, nucleic acid encoding NANOG, and nucleic acid encoding LIN28.
Dependencies are inferred from the claim text.