Generation of plants with altered oil content

A feed, meal, grain, food, or seed comprising a polypeptide:

• encoded by the nucleic acid sequence as set forth in SEQ ID NO:
• 1;
• having the amino acid sequence set forth in SEQ ID NO:
• 2;
• or
• having an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:
• 2, wherein the feed, meal, grain, food, or seed has at least 3.5% greater oil content relative to meal, grain, food, or seed that does not comprise the polypeptide.

A transgenic plant with a high oil phenotype made by a method comprising:

• introducing into progenitor cells of the plant a heterologous constitutive promoter operatively linked to a heterologous polynucleotide that encodes a HIO32.2 polypeptide, to produce transformed cells overexpressing the HIO32.2 polypeptide, wherein the HIO32.2 polypeptide has an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:
• 2 and conveys a high oil phenotype on a plant when expressed in said plant;
• growing the transformed progenitor cells to produce a transgenic plant;
• and
• identifying the high oil phenotype in said transgenic plant by measuring oil content of the transgenic plant and selecting a transgenic plant that possesses at least 3.5% greater seed oil content relative to a plant of the same species that does not comprise the heterologous constitutive promoter operatively linked to the heterologous polynucleotide;
• thereby producing the transgenic plant with a high oil phenotype.