Microorganism producing inosine and method of producing inosine using the same
AssigneeCj Cheiljedang Corporation
InventorsYoung-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
Life of this patent
Estimating the term…
Abstract
The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
Claims4
A recombinant Corynebacterium ammoniagenes, producing inosine by inactivation of the gene encoding nucleoside hydrolase II having the nucleotide sequence represented by SEQ ID NO:
- 1 and by over-expression of the gene encoding 5′-nucleotidase represented by SEQ ID NO:
- 2.
A recombinant Corynebacterium ammoniagenes, which is derived from Corynebacterium ammoniagenes CJIP2401 (KCCM-10610), producing inosinic acid and inosine by inactivation of the gene encoding nucleoside hydrolase II having the nucleotide sequence represented by SEQ ID NO:1, wherein the recombinant Corynebacterium ammoniagenes is Corynebacterium ammoniagenes CJIG650 (KCCM-101828P).
Dependencies are inferred from the claim text.