Process for the production of organic formulation of bio-pesticide Pseudomonas fluorescens
AssigneesDepartment of Biotechnology, Indian Institute of Horticultural Research
InventorMahendrakar Sreenivasa Rao
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Abstract
This invention relates to a process for the production of organic formulation of bio pesticide containing Pseudomonas Fluorescens comprising preparation of Mother culture of P. fluorescens and inoculating in King's B broth, at 30±1° C. for 24-36 hours followed by liquid fermentation process in 5-10% pongamia cake aqueous extract, 5-10% neem cake aqueous extract, 0.3-0.5% sugarcane molasses and King's B broth followed by solid fermentation using sterile Pongamia deoiled cake, Neem deoiled cake, wheat Bran.
Claims4
A process for the production of organic formulation of biopesticide containing Pseudomonas fluorescens comprising:
- a first step of preparing a Mother culture of P. fluorescens and inoculating in King's B broth, at 30±1° C. for 24-36 hours;
- a second step of liquid fermentation of the culture obtained through inoculating the Mother culture in King's B broth in a first substrate comprising 5-10% pongamia cake aqueous extract, 5-10% neem cake aqueous extract, 0.3-0.5% sugarcane molasses and King's B broth;
- followed by a third step of solid fermentation of the culture obtained through liquid fermentation in a second substrate comprising sterile Pongamia deoiled cake, Neem deoiled cake, and Wheat Bran.
Dependencies are inferred from the claim text.