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US 8,153,762 B2Granted
Composition for palladium-mediated cleavage of peptides containing CXC, CXH or CHM sequences
AssigneeMedtronic, Inc.
InventorsBarton Holmquist, Daniel J. Strydom, Jin Seog Seo
Life of this patent
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Abstract
The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
Claims27
A composition comprising:
- a palladium complex selected from the group consisting of a palladium (II) complex and a palladium (IV) complex;
- an acidic medium;
- and
- a substrate polypeptide comprising a desired polypeptide and the sequence X1-Cys-X2-X3, wherein
- X1 is the amino acid at the carboxyl-terminus of the desired peptide,
- X1 and X2 can be any amino acid, and
- X3 is selected from the group consisting of cysteine, histidine, and methionine.
4 more elements
A composition comprising:
- a substrate polypeptide having (i) a first cleavage site that links the N-terminus of a first amino acid sequence defining a desired peptide to the carboxyl terminus of a second amino acid sequence defining a leader sequence, and (ii) a second X1-cysteine cleavage site, wherein X1 is the amino acid at the peptide carboxy-terminus and the cysteine is the first amino acid of a tail sequence comprising the sequence cysteine -X2-X3, where X2 is any amino acid and X3 is an amino acid selected from the group consisting of cysteine, histidine, and methionine, wherein the first and second cleavage sites are cleavable by a palladium complex in an acidic medium;
- a palladium complex selected from the group consisting of a palladium(II) complex and a palladium(IV) complex;
- and
- an acidic organic solvent at a concentration of between about 2 to about 22 molar.
1 more element
Dependencies are inferred from the claim text.