Back to results
US 8,153,389 B2Granted
Methods for the identification of PI3K interacting molecules and for the purification of PI3K
AssigneeCellzone AG
InventorsGiovanna Bergamini Moore, Andrew Cansfield, Nigel Ramsden, Gitte Neubauer
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for the identification of a PI3K interacting compound using phenylthiazole ligand 1.
Claims21
A method for the identification of a phosphoinositide 3-kinase (PI3K) interacting compound, comprising the steps of
- a) providing a protein preparation containing PI3K,
- b) contacting the protein preparation with phenylthiazole ligand 1 immobilized on a solid support under conditions allowing the formation of a phenylthiazole ligand 1-PI3K complex,
- c) incubating the phenylthiazole ligand 1-PI3K complex with a given compound, and
- d) determining whether the compound is able to separate PI3K from the immobilized phenylthiazole ligand 1,
- thereby identifying the PI3K interacting compound.
2 more elements
A method for the identification of a PI3K interacting compound, comprising the steps of
- a) providing a protein preparation containing PI3K,
- b) contacting the protein preparation with phenylthiazole ligand 1 immobilized on a solid support and with a given compound under conditions allowing the formation of a phenylthiazole ligand 1-PI3K complex, and
- c) detecting the phenylthiazole ligand 1-PI3K complex formed in step b), wherein a reduced amount of the phenylthiazole ligand 1-PI3K complex formed in the presence of the compound in comparison to the amount of phenylthiazole ligand 1-PI3K complex formed in the absence of the compound indicates that the compound is a PI3K interacting compound.
A method for the identification of a PI3K interacting compound, comprising the steps of:
- a) providing two aliquots of a protein preparation containing PI3K,
- b) contacting one aliquot with the phenylthiazole ligand 1 immobilized on a solid support under conditions allowing the formation of a phenylthiazole ligand 1-PI3K complex,
- c) contacting the other aliquot with the phenylthiazole ligand 1 immobilized on a solid support and with a given compound under conditions allowing the formation of a phenylthiazole ligand 1-PI3K complex, and
- d) determining the amount of the phenylthiazole ligand 1-PI3K complex formed in steps b) and c), wherein a reduced amount of the phenylthiazole ligand 1-PI3K complex formed in step (c) in comparison to that formed in step (b) indicates that the compound is a PI3K interacting compound.
1 more element
A method for the identification of a PI3K interacting compound, comprising the steps of:
- a) providing two aliquots each comprising at least one cell containing PI3K,
- b) incubating one aliquot with a given compound,
- c) harvesting the cells of each aliquot,
- d) lysing the cells of each aliquot in order to obtain protein preparations,
- e) contacting the protein preparations of each aliquot with the phenylthiazole ligand 1 immobilized on a solid support under conditions allowing the formation of a phenylthiazole ligand 1-PI3K complex, and
- f) determining the amount of the phenylthiazole ligand 1-PI3K complex formed in each aliquot in step e), wherein a reduced amount of the phenylthiazole ligand 1-PI3K complex formed in the aliquot incubated with the compound in comparison to the aliquot not incubated with the compound indicates that the compound is a PI3K interacting compound.
3 more elements
Dependencies are inferred from the claim text.