# US 8,148,514 B2 · hTERT gene expression regulatory gene > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,148,514 B2 - Document ID: US8148514B2 - Status: Granted - Kind: B2 - Application number: 12/751,226 - Filed: 2010-03-31 - Published: 2010-07-22 - Granted: 2012-04-03 - Earliest claimed: 2005-04-15 - Assignees: National University Corporation Tottori University - Inventors: Norimasa Miura - Web page: https://patentgrep.com/patent/US8148514B2 ## CPC - A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy - A61P 35/00: Antineoplastic agents - C07K 14/4702: {Regulators; Modulating activity} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract Disclosed is a novel substance capable of regulating the expression of a telomerase reverse transcriptase gene in a cell of a mammal. A gene capable of regulating the expression of hTERT, comprising a nucleotide sequence depicted in SEQ ID No: 1 or 2. The expression of a telomerase reverse transcriptase gene can be inhibited by inhibiting the expression of the gene. By utilizing this mechanism, the expression of a telomerase reverse transcriptase gene can be regulated. ## Claims ### Claim 1 A double stranded RNA comprising: a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA; and a second base sequence with a length of 15 bases or longer and 30 bases or shorter, complementary to the first base sequence; wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of: (a) the base sequence shown in SEQ ID No. 4; and (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4; and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to suppress expression of the hTERT expression regulatory RNA. ### Claim 2 (depends on 1) The double stranded RNA recited in claim 1, wherein, the first base sequence is the base sequence recited in any one of SEQ ID No. 20, 22, and 24 through 32. ### Claim 3 (depends on 1) The double stranded RNA recited in claim 1, wherein, the first base sequence comprises an overhang portion on the 3′ end with a length of 2 bases, and the second base sequence comprises an overhang portion on the 3′ end with a length of two bases. ### Claim 4 (depends on 1) A complex comprising the double stranded RNA recited in claim 1 and a promoter substance for incorporation into a cell. ### Claim 5 (depends on 1) The double stranded RNA recited in claim 1, wherein the double stranded RNA has been designed and constructed. ### Claim 6 (depends on 5) The double stranded RNA recited in claim 5, wherein, the first base sequence is the base sequence recited in any one of SEQ ID No. 20, 22, and 24 through 32. ### Claim 7 (depends on 5) The double stranded RNA recited in claim 5, wherein, the first base sequence comprises an overhang portion on the 3′ end with a length of 2 bases, and the second base sequence comprises an overhang portion on the 3′ end with a length of two bases. ### Claim 8 (depends on 5) A complex comprising the double stranded RNA recited in claim 5 and a promoter substance for incorporation into a cell. ### Claim 9 A single stranded RNA comprising: a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA; and a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence; wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of: (a) the base sequence shown in SEQ ID No. 4; and (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4; and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to suppress expression of the hTERT expression regulatory RNA. ### Claim 10 (depends on 9) The single stranded RNA recited in claim 9, wherein, between the first base sequence and the second base sequence, a hairpin loop sequence of 4 bases or more is included. ### Claim 11 (depends on 9) The single stranded RNA recited in claim 9, wherein the single stranded RNA has been designed and constructed. ### Claim 12 (depends on 11) The single stranded RNA recited in claim 11, wherein, between the first base sequence and the second base sequence, a hairpin loop sequence of 4 bases or more is included. ### Claim 13 An hTERT expression regulatory gene expression vector, comprising a DNA sequence and a vector, wherein the DNA sequence encodes a single stranded RNA that consists of: a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA; a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence; and a hairpin loop sequence of 4 bases or more; wherein the hairpin loop sequence is located between the first base sequence and the second base sequence; and the hTERT expression regulatory RNA comprises a member selected from the group consisting of: (a) the base sequence shown in SEQ ID No. 4; and (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4; and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to allow the single stranded RNA to suppress expression of the hTERT expression regulatory RNA. ### Claim 14 An RNA composition comprising a plurality of types of double stranded RNA formed by cleaving, with a micro RNA maturation enzyme, a precursor double stranded RNA that comprises: an hTERT expression regulatory RNA, and an RNA comprising a base sequence complementary to the hTERT expression regulatory RNA, wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of: (a) the base sequence shown in SEQ ID No. 4; and (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4. ### Claim 15 (depends on 14) The RNA composition recited in claim 14, wherein the precursor double stranded RNA is an isolated and purified RNA.