# US 8,148,140 B2 · Target substance detecting element, target substance detecting apparatus, and target substance detecting method

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- Number: US 8,148,140 B2
- Document ID: US8148140B2
- Status: Granted
- Kind: B2
- Application number: 12/294,611
- Filed: 2007-05-11
- Published: 2009-04-23
- Granted: 2012-04-03
- Earliest claimed: 2006-05-12
- Assignees: Canon Kabushiki Kaisha
- Inventors: Junta Yamamichi; Tetsunori Ojima
- Web page: https://patentgrep.com/patent/US8148140B2

## CPC

- G01N 33/54306: {Solid-phase reaction mechanisms}
- G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)}
- G01N 33/553: Metal or metal coated
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention enables to classify and measure two or more kinds of target substances which have the same recognition site recognized by a specific capturing body. Specifically, by using a target substance detecting element for detecting two or more targets, whose kinds are different mutually, at the same time, the target substance detecting element, characterized by having a base material, two or more kinds of metal structures provided on a surface of the base material, and a target capturing body provided on each surface of the two or more kinds of metal structures, and in that the number of kinds of the metal structures are equal to or more than the number of kinds of the target substances, concentrations of the two or more kinds of target substances can be calculated by integrating and analyzing detection signals detected from the target substance detecting element.

## Claims

### Claim 1

A target substance detecting apparatus using localized surface plasmon resonance, which detects kinds of target substances which have the same recognition site recognized by a specific capturing body but whose kinds are different mutually, comprising a target substance detecting element, a reaction mechanism for contacting a sample containing a target substance with the target substance detecting element, a light source for irradiating the target substance detecting element with light, a detecting unit for detecting a detection signal from the target substance detecting element, and an analyzing unit for analyzing the detection signal to determine the target substance,
the target substance detecting element comprising a base material, two or more kinds of metal structures which are mutually different in shape, material or size and which give rise to localized surface plasmon resonance by receiving the light, provided on a surface of the base material, and a target capturing unit provided on each surface of the metal structures;
the detecting unit acquiring a spectrum of outgoing light from the target substance detecting element contacted with the sample, classifying the acquired spectrum according to kind of the metal structures to determine spectral peaks, and detecting a change of the spectral peak of each kind of the metal structures from the state before the contact with the sample to the state after the contact as the detection signal; and
the analyzing unit integrating and analyzing the detected detection signals to calculate the concentration of each kind of the target substances.

### Claim 2 (depends on 1)

The target substance detecting apparatus according to claim 1, wherein the number of kinds of the metal structures are equal to or more than the number of kinds of the target substances.

### Claim 3 (depends on 1)

The target substance detecting apparatus according to claim 1, wherein the analyzing unit uses simultaneous equations with several unknowns to calculate the concentration.

### Claim 4 (depends on 1)

The target substance detecting apparatus according to claim 1, wherein the analyzing unit calculates the concentration from data for a calibration which has been obtained beforehand through solutions of the target substance in known concentrations.