Diagnostics and vaccines for mycobacterial infections of animals and humans
AssigneeHAV Vaccines Limited
InventorsJohn Hermon-Taylor, Timothy John Bull
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Abstract
This invention relates to the protein, Mycobacterium paratuberculosis acylase (mpa) and the gene encoding mpa, which we have identified in the pathogen Mycobacterium paratuberculosis Mptb (also designated Mycobacterium avium subspecies paratuberculosis MAP), and to their use in the diagnosis of Mptb/MAP infections in animals and humans, as well as their use as components of vaccines for the prevention and treatment of diseases caused by Mptb/MAP. The importance of an intact uninterrupted mpa gene as a determinant of pathogenicity in Mptb/MAP is recognized and the invention also provides attenuated strains of normally pathogenic Mptb/MAP and other mycobacteria in which mpa has been inactivated, for use as vaccines.
Claims8
An expression vector which comprises:
- (a) a polynucleotide comprising the entire nucleotide sequence set out in SEQ ID NO. 1 or the complement thereof;
- or
- (b) a polynucleotide that encodes a polypeptide that comprises:
- (i) the sequence set out in SEQ ID NO:
- 2;
- (ii) the polypeptide encoded by nucleotides 210-1335 of SEQ ID NO:
- 1;
- (iii) amino acids 158-211 of SEQ ID NO:
- 2;
- or
- (iv) amino acids 380-444 of SEQ ID NO:
- 2,
- wherein said polypeptide has the ability to stimulate an immune response against the polypeptide of SEQ ID NO:
- 2;
- operably linked to regulatory sequences capable of directing expression of said polynucleotide in a host cell.
An isolated polynucleotide selected from:
- (a) a polynucleotide comprising the entire nucleotide sequence set out in SEQ ID No. 1 or the complement thereof;
- or
- (b) a polynucleotide which encodes the polypeptide of SEQ ID NO:
- 2,
- wherein said polynucleotide encodes a polypeptide that has the ability to stimulate an immune response against the polypeptide of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.