Regulation of autophagy pathway phosphorylation and uses thereof
AssigneeAbgent
InventorsChun Wu, John A. Mountzouris, Bingren Hu, Chunli Liu
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Abstract
The invention relates to polypeptides and proteins known to function in the autophagy pathway that have novel phosphorylation sites. The invention also relates to antibodies specific to these polypeptides and proteins that are phosphorylated or not phosphorylated at novel phosphorylated sites. The invention also relates to methods of producing these antibodies and use of these antibodies in the treatment of diseases related to autophagocytosis.
Claims6
A method for detecting an autophagy protein or fragment comprising an amino acid sequence TNXGE (SEQ ID NO:1377), wherein the x residue is phosphorylated or nonphosphorylated serine, in a sample, which method comprises:
- (a) contacting a sample containing or suspected of containing an autophagy protein or fragment comprising said amino acid sequence with an isolated antibody that specifically binds to an epitope that comprises the amino acid residue x in said amino acid sequence, wherein the x residue is phosphorylated or nonphosphorylated serine;
- and
- (b) assessing a complex formed between said autophagy protein or fragment, if present in said sample, and said antibody, to determine the presence, absence and/or amount of said autophagy protein or fragment in said sample.
Dependencies are inferred from the claim text.