# US 8,147,774 B2 · Micro passage chip and fluid transferring method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,147,774 B2 - Document ID: US8147774B2 - Status: Granted - Kind: B2 - Application number: 12/307,403 - Filed: 2007-07-04 - Published: 2009-08-13 - Granted: 2012-04-03 - Earliest claimed: 2006-07-05 - Assignees: Aida Engineering, Ltd. - Inventors: Hisashi Hagiwara; Yoshinori Mishina - Web page: https://patentgrep.com/patent/US8147774B2 ## CPC - B01J 19/0093: {Microreactors, e.g. miniaturised or microfabricated reactors (laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027)} - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - F04B 43/043: {Micropumps} - B01J 2219/00783: Laminate assemblies, i.e. the reactor comprising a stack of plates - B01J 2219/00822: Metal - B01J 2219/00831: Glass - B01J 2219/00833: Plastic - B01J 2219/00835: Comprising catalytically active material - B01J 2219/00837: comprising coatings other than catalytically active coatings - B01J 2219/0086: Dimensions of the flow channels - B01J 2219/00889: Mixing - B01J 2219/00891: Feeding or evacuation - B01L 2200/12: Specific details about manufacturing devices - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution - B01L 2300/0887: Laminated structure - B01L 2300/12: Specific details about materials - B01L 2400/0481: squeezing of channels or chambers - B01L 2400/0633: with moving parts - B01L 2400/0672: Swellable plugs - Y10T 137/0318: Processes - Y10T 137/0324: With control of flow by a condition or characteristic of a fluid - Y10T 137/0352: Controlled by pressure - Y10T 137/0396: Involving pressure control - Y10T 137/1624: Destructible or deformable element controlled - Y10T 428/24612: Composite web or sheet - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - F04B: POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS (machines for liquids, or pumps, of rotary-piston or oscillating-piston type F04C; non-positive-displacement pumps F04D; pumping of fluid by direct contact of another fluid or by using inertia of fluid to be pumped F04F) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract There is provided by the present invention a novel micro passage chip having such a structure that a fluid can be transferred without using a physical or mechanical squeezing means that is applied from above the substrate of the micro passage chip. The micro passage chip of the present invention comprises at least a first substrate, a second substrate, and an intermediate substrate interposed between the first substrate and the second substrate and is characterized in that a first non-adhesive thin-film layer is formed on at least one of the mating sides of the first substrate and the intermediate substrate and at least one port for fluids that is in contact with the first non-adhesive thin-film layer and which is open to the outer surface of the first substrate is provided in any position on the first non-adhesive thin-film layer, and that at least a portion of a second non-adhesive thin-film layer whose length is either the same as or different from that of the first non-adhesive thin-film layer is formed on at least one of the mating sides of the second substrate and the intermediate substrate such that it lies either above or under the first non-adhesive thin-film layer, with the intermediate substrate lying in between, and a pressurizing port that is in contact with the second non-adhesive thin-film layer and which is open to the outer surface of the first or the second substrate is provided in at least one area on the second non-adhesive thin-film layer. ## Claims ### Claim 1 A micro passage chip comprising at least a first substrate of an elastic material, a second substrate, and an intermediate substrate of an elastic material interposed between the first substrate and the second substrate, further comprising: a first non-adhesive thin-film layer is formed on at least one of the mating sides of the first substrate and the intermediate substrate and at least one port for fluids that is in contact with the first non-adhesive thin-film layer and which is open to the outer surface of the first substrate is provided in any position on the first non-adhesive thin-film layer, a second non-adhesive thin-film layer whose length is either the same as or different from that of the first non-adhesive thin-film layer is formed on at least one of the mating sides of the second substrate and the intermediate substrate such that it lies either above or under the first non-adhesive thin-film layer, and parallel thereto, with the intermediate substrate lying in between, and a pressurizing port that is in contact with the second non-adhesive thin-film layer and which is open to the outer surface of the first or the second substrate is provided in at least one area on the second non-adhesive thin-film layer; a first non-adhesive part disposed in an interface area between the first substrate and the intermediate substrate where the first non-adhesive thin-film layer is formed; a second non-adhesive part disposed in a second interface area between the second substrate and the intermediate substrate where the second non-adhesive thin-film layer is formed; wherein under a non-pressure state at the pressurizing port, both the first non-adhesive part and the second non-adhesive part have no capacity, and wherein under a positive pressure state at the pressurizing port, the first substrate corresponding to the first non-adhesive part deforms to form a channel for the fluids, and the intermediate substrate corresponding to the second non-adhesive part deforms to form a microchannel adapted to squeeze the fluids through the channel. ### Claim 2 (depends on 1) The micro passage chip according to claim 1, wherein the first non-adhesive thin-film layer includes, halfway down it, at least one enlarged region layer having at least one planar shape selected from the group consisting of a circular, an elliptical, a rectangular, and a polygonal shape. ### Claim 3 (depends on 1) The micro passage chip according to claim 1, wherein the first non-adhesive thin-film layer and the second non-adhesive thin-film layer each have a film thickness in the range of 10 nm to 300 μm and a width in the range of 10 μm to 3000 μm. ### Claim 4 A micro passage chip comprising at least a first substrate, a second substrate, and an intermediate substrate, of an elastic material, interposed between the first substrate and the second substrate, further comprising: a hollow, concave channel through which to pass a fluid is formed on the side of the first substrate that is adhered to the intermediate substrate and at least one port for fluids that communicates with the concave channel and which is open to the outer surface of the first substrate is provided at any position in the concave channel, a second non-adhesive thin-film layer whose length is either the same as or different from that of the concave channel is formed on at least one of the mating sides of the second substrate and the intermediate substrate such that it lies either above or under the concave channel, and parallel thereto, with the intermediate substrate lying in between, and a pressurizing port that is in contact with the second non-adhesive thin-film layer and which is open to the outer surface of the first or second substrate is provided in at least one area on the second non-adhesive thin-film layer; a second non-adhesive part disposed in an interface area between the second substrate and the intermediate substrate where the second non-adhesive thin-film layer is formed; wherein under a non-pressure state at the pressurizing port, the second non-adhesive part has no capacity, and wherein under a positive pressure state at the pressurizing port, the intermediate substrate corresponding to the second non-adhesive part deforms to form a microchannel adapted to squeeze the fluids through the concave channel. ### Claim 5 (depends on 4) The micro passage chip according to claim 4, wherein the second non-adhesive thin-film layer has a film thickness in the range of 10 nm to 300 μm and a width in the range of 10 μm to 3000 μm. ### Claim 6 A micro passage chip comprising at least a first substrate, a second substrate, and a first and a second intermediate substrate that are interposed between the first substrate and the second substrate, wherein a first non-adhesive thin-film layer is formed on at least one of a mating side of the first and the second intermediate substrate and at least one port for fluids that is in contact with the first non-adhesive thin-film layer and which is open to the outer surface of the first substrate is provided in any position on the first non-adhesive thin-film layer, at least a portion of a second non-adhesive thin-film layer whose length is either the same as or different from that of the first non-adhesive thin-film layer is formed on at least one of the mating sides of the second substrate and the second intermediate substrate such that it lies either above or under the first non-adhesive thin-film layer, with the second intermediate substrate lying in between, and a first pressurizing port that is in contact with the second non-adhesive thin-film layer and which is open to the outer surface of the first or the second substrate is provided in at least one area on the second non-adhesive thin-film layer, and at least a portion of a third non-adhesive thin-film layer whose length is either the same as or different from that of the first non-adhesive thin-film layer is formed on at least one of the mating sides of the first substrate and the first intermediate substrate such that it lies either above or under the first non-adhesive thin-film layer, with the first intermediate substrate lying in between, and a second pressurizing port that is in contact with the third non-adhesive thin-film layer and which is open to the outer surface of the first or the second substrate is provided in at least one area on the third non-adhesive thin-film layer. ### Claim 7 (depends on 6) The micro passage chip according to claim 6, wherein a first non-adhesive part is present in the area of the interface between the first and the second intermediate substrate where the first non-adhesive thin-film layer is formed, a second non-adhesive part is present in the area of the interface between the second substrate and the second intermediate substrate where the second non-adhesive thin-film layer is formed, a third non-adhesive part is present in the area of the interface between the first substrate and the first intermediate substrate where the third non-adhesive thin-film layer is formed, the first non-adhesive part serves as a channel for the fluid, and the second and third non-adhesive parts serve as squeezing means for transferring the fluid. ### Claim 8 (depends on 6) The micro passage chip according to claim 6, wherein the first non-adhesive thin-film layer includes, halfway down it, at least one enlarged region layer having at least one planar shape selected from the group consisting of a circular, an elliptical, a rectangular, and a polygonal shape. ### Claim 9 (depends on 6) The micro passage chip according to claim 6, wherein the first non-adhesive thin-film layer, the second non-adhesive thin-film layer, and the third non-adhesive thin-film layer each have a film thickness in the range of 10 nm to 300 μm and a width in the range of 10 μm to 3000 μm. ### Claim 10 (depends on 1) The micro passage chip according to claim 1, wherein the first substrate is made of polydimethylsiloxane (PDMS), the second substrate is made of polydimethylsiloxane (PDMS) or glass, and the intermediate substrate(s) is(are) made of polydimethylsiloxane (PDMS).