Pantothenate kinase overexpression and pantothenic acid supplementation in actinomycetes
AssigneeRice University
InventorsHei Chan Lee, Jae Kyung Sohng, Ka-Yiu San
Life of this patent
Estimating the term…
Abstract
The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives increased production of secondary metabolites, such as polyketides.
Claims17
An engineered Actinomycetes cell comprising an overexpression of a recombinant pantothenate kinase (panK) gene wherein the overexpression of said recombinant panK gene increases pantothenate kinase activity and polyketide synthesis and increases acetyl-coA levels 2 fold greater than in Actinomycete cells without said overexpressed panK gene.
A method of increasing polyketide production in an Actinomycetes cell comprising:
- a) producing an engineered Actinomycete cell that overexpresses a recombinant pantothenate kinase (panK) gene,
- b) selecting an engineered Actinomycete cell that has chromosomal integration of said recombinant panK gene;
- and
- c) growing said engineered and selected Actinomycete cell in a medium supplemented with pantothenic acid,
- wherein said engineered and selected Actinomycete cell produces more polyketide than a wild type Actinomycete grown in said medium and wherein acetyl-coA is increased 2 fold greater than in Actinomycete cells without said overexpressed panK gene.
A method of increasing polyketide production in an Actinomycetes bacteria comprising:
- a) transforming an Actinomycete bacteria with a vector that encodes a pantothenate kinase (panK) gene,
- b) selecting a transformed Actinomycete bacteria that has chromosomal integration of said panK gene;
- and
- c) growing said transformed and selected Actinomycete bacteria under conditions that allow overexpression of said panK gene and in a medium supplemented with pantothenic acid,
- wherein said transformed Actinomycete bacteria produces more polyketide than a non-transformed Actinomycete bacteria grown in said medium and wherein acetyl-coA is increased 2 fold greater than in Actinomycete cells without the panK gene.
Dependencies are inferred from the claim text.