Real-time monitoring apparatus for biochemical reaction

A real-time monitoring apparatus for biochemical reaction, comprising:

• a thermoelectric element capable of supplying heat into reaction tubes;
• a heat transmission block which transmits the heat to the reaction tubes;
• a tube plate capable of holding a sample;
• a lamp for irradiating to a sample contained in at least one of the reaction tubes in the tube plate;
• at least one reflective mirror;
• an optical waveguide positioned in front of the lamp which has an open structure in cooperation with said reflective mirror, said optical waveguide having a configuration that alters a light path passing through at least one end of the optical waveguide and provides a uniform intensity of light;
• an infra-red cutting filter filtering light transmitted through a light path that comprises said infra-red cutting filter, said reflective mirror and the optical waveguide and said infra-red cutting filter cutting infra-red from the lamp and a selective transmission filter for transmitting light selectively to monitor a reaction progress;
• said light transmitted through a light path illuminating the sample with a uniform light intensity distribution as provided by the uniform intensity of light from the optical waveguide, the optical waveguide reducing of the difference in light intensity between the center and the edges of the tube plate;
• a condensing lens positioned outside of a portion of a light path comprising said reflective mirror, the optical waveguide and the infra-red cutting filter;
• an optical system comprising a receiving part for receiving fluorescence transmitted through a second focusing lens, using a light receiving element capable of receiving the fluorescence, the fluorescence irradiated from the sample by light emitted from a light irradiation source as transmitted through a light path comprising the optical waveguide, the selective transmission filter and a first focusing lens;
• and
• said components arranged so that light will travel through optical components of the real time monitoring apparatus in the order of the lamp, the infra-red cutting filter, the optical waveguide, the selective transmission filter, the first focusing lens through the sample and the light receiving element.