Human T1R2 nucleic acid sequences and polypeptides
AssigneeSenomyx, Inc.
InventorsJon Elliot Adler, Xiaodong Li, Lena Staszewski, Shawn O'Connell, Sergey Zozulya
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Abstract
Binding assays for identifying compounds that modulate human T1R2 polypeptide associated taste are disclosed. These assays detect the specific binding of compounds to a human T1R2 polypeptide or the modulation of the specific binding of a compound that specifically binds to a human T1R2 polypeptide. The binding assays may include the use of detectable labels, e.g., radionuclides, enzymes, fluorophases, and the like. Compounds identified in these binding assays have putative application as T1R2 taste modulators, particularly sweet taste, and potentially are useful additives in compositions for human or animal consumption.
Claims13
An isolated nucleic acid that encodes a T1R2 taste receptor polypeptide or T1R2 extracellular or T1R2 cytoplasmic domain wherein said nucleic acid is selected from the group consisting of (i) the nucleic acid of SEQ ID NO 23;
- (ii) a nucleic acid encoding a polypeptide having at least 90% sequence identity to the T1R2 polypeptide in SEQ ID NO:21 or an extracellular or cytoplasmic domain thereof;
- (iii) a nucleic acid s encoding a human T1R2 polypeptide or a T1R2 cytoplasmic or extracellular domain wherein said nucleic acid hybridizes under stringent hybridization conditions to the complement of the nucleic acid sequence contained in SEQ ID NO:
- 23 and wherein said stringent hybridization conditions are hybridization in 50% formamide, 5 times SSC and 1% SDS, incubating at 42 degrees C., with wash in 0.2 times SSC and 0.1% SDS at 65 degrees C., wherein the hybridization and wash steps are each carried out for at least 1 minute.
Dependencies are inferred from the claim text.